Rhox13^tm1.2Chge
Targeted Allele Detail

Summary

• Symbol: Rhox13^tm1.2Chge
• Name: reproductive homeobox 13; targeted mutation 1.2, Christopher B Geyer
• MGI ID: MGI:5902138
• Gene: Rhox13 Location: ChrX:37210096-37218844 bp, + strand Genetic Position: ChrX, 22.38 cM
• Alliance: Rhox13^tm1.2Chge page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:237048
• Parent Cell Line: E14TG2a.4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This allele is derived from the "knockout first" vector used to generate the Rhox13 allele. The vector is constructed as follows. The L1L2_Bact_P cassette was inserted at position 38122599 of Chromosome X upstream of exon 2 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by the neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 38123363. Exon 2 is thus flanked by loxP sites. A conditional-ready (floxed) allele was created by subsequent flp-mediated recombination which removes the lacZ and neo cassettes, leaving exon 2 floxed. Finally, exon 2 was deleted through cre-mediated recombination, creating a null allele. (J:237048)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rhox13 Mutation: 9 strains or lines available

References

• Original: J:237048 Busada JT, et al., Rhox13 is required for a quantitatively normal first wave of spermatogenesis in mice. Reproduction. 2016 Nov;152(5):379-88
• All: 1 reference(s)