Pdgfrb^{tm1.1(cre/ERT2)Csln}
Targeted Allele Detail

Summary

• Symbol: Pdgfrb^{tm1.1(cre/ERT2)Csln}
• Name: platelet derived growth factor receptor, beta polypeptide; targeted mutation 1.1, Chyuan-Sheng Lin
• MGI ID: MGI:5901894
• Synonyms: PDGFRbata-P2A-CreERT2
• Gene: Pdgfrb Location: Chr18:61178222-61218133 bp, + strand Genetic Position: Chr18, 34.41 cM
• Alliance: Pdgfrb^{tm1.1(cre/ERT2)Csln} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:263753
• Parent Cell Line: KV1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Pdgfrb^{tm1.1(cre/ERT2)Csln} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Pdgfrb (MGI:97531)

• Mutation details: A targeting vector was designed to replace the translation stop codon of the platelet derived growth factor receptor, beta polypeptide (Pdgfrb) gene with a creER fusion gene (a Cre recombinase fused to a human estrogen receptor ligand binding domain) followed by a frt-flanked neomycin resistance (neo) cassette. A viral P2A oligopeptide, from porcine teschovirus-1, was inserted upstream of the creER fusion gene to ribosomal skipping. The construct was electroporated into (C57BL/6J x 129S6/SvEvTac)F1-derived KV1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL6/N blastocysts and resulting chimeric males were bred to Tg(ACTFLPe)9205Dym/J females to remove the neo cassette. These mice express Cre-ER protein in pericytes in the vasculature of organs including brain and retina. Restricted to the cytoplasm, Cre-ER can only gain access to the nuclear compartment after exposure to tamoxifen. (J:263753)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Pdgfrb (mouse) Summary of all recombinase alleles driven by Pdgfrb. Comparative matrix view of recombinase activities Alliance mouse Pdgfrb gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pdgfrb Mutation: 84 strains or lines available

References

• Original: J:263753 Cuervo H, et al., PDGFRbeta-P2A-CreER(T2) mice: a genetic tool to target pericytes in angiogenesis. Angiogenesis. 2017 Nov;20(4):655-662
• All: 15 reference(s)