Scai^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Scai^{tm1b(EUCOMM)Hmgu}
• Name: suppressor of cancer cell invasion; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5898410
• Synonyms: SCAI^-
• Gene: Scai Location: Chr2:38956226-39080746 bp, - strand Genetic Position: Chr2, 24.42 cM
• Alliance: Scai^{tm1b(EUCOMM)Hmgu} page
• IMPC: Scai gene page

Mutation origin

• Mutant Cell Line: HEPD0516_1_G04
• Germline Transmission: Earliest citation of germline transmission: J:240463
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 38997948 of Chromosome 2 upstream of critical exon 7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 7 at position 38998905. Exon 7 is thus flanked by loxP sites. The exon and neo cassette were deleted through subsequent cre-mediated recombination. (J:157065, J:240463)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Scai Mutation: 33 strains or lines available

References

• Original: J:240463 Hansen RK, et al., SCAI promotes DNA double-strand break repair in distinct chromosomal contexts. Nat Cell Biol. 2016 Dec;18(12):1357-1366
• All: 2 reference(s)