Pdgfa^tm1Vlcg
Targeted Allele Detail

Summary

• Symbol: Pdgfa^tm1Vlcg
• Name: platelet derived growth factor, alpha; targeted mutation 1, Velocigene
• MGI ID: MGI:5897007
• Synonyms: Pdgfa^ex4COIN, Pdgfa^fl
• Gene: Pdgfa Location: Chr5:138961769-138983125 bp, - strand Genetic Position: Chr5, 77.65 cM
• Alliance: Pdgfa^tm1Vlcg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:240991
• Parent Cell Line: F1H4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NTac)F1

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Exon 4 was targeted with the insertion of a COIN (conditional by inversion) cassette. The cassette consists of a splice acceptor site and a LacZ gene with polyA site flanked by lox71 and lox66 sites in head-to-head orientation. The cassette is inserted in inverted orientation compared to the target gene. Upon cre-mediated recombination the modified loxP sites will mediate irreversible inversion of the cassette and thus create a gene for a fusion between the 5' end of the target gene and the lacZ gene. Also inserted into exon 4, downstream of the COIN cassette but still in exon 4, was an FRT site flanked neomuycin resistance gene cassette and a splice acceptor site. This allele is conditional-ready and splits exon 4 into exon 4a and 4b which has no effect on the mature transcript. (J:240991)

Expression

In Mice Carrying this Mutation:

191 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdgfa Mutation: 20 strains or lines available

References

• Original: J:240991 Andrae J, et al., Characterization of platelet-derived growth factor-A expression in mouse tissues using a lacZ knock-in approach. PLoS One. 2014;9(8):e105477
• All: 6 reference(s)