About   Help   FAQ
Pdgfatm1Vlcg
Targeted Allele Detail
Summary
Symbol: Pdgfatm1Vlcg
Name: platelet derived growth factor, alpha; targeted mutation 1, Velocigene
MGI ID: MGI:5897007
Synonyms: Pdgfaex4COIN, Pdgfafl
Gene: Pdgfa  Location: Chr5:138961769-138983125 bp, - strand  Genetic Position: Chr5, 77.65 cM
Alliance: Pdgfatm1Vlcg page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:240991
Parent Cell Line:  F1H4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NTac)F1
Mutation
description
Allele Type:    Targeted (Conditional ready, No functional change)
Mutation:    Insertion
 
Mutation detailsExon 4 was targeted with the insertion of a COIN (conditional by inversion) cassette. The cassette consists of a splice acceptor site and a LacZ gene with polyA site flanked by lox71 and lox66 sites in head-to-head orientation. The cassette is inserted in inverted orientation compared to the target gene. Upon cre-mediated recombination the modified loxP sites will mediate irreversible inversion of the cassette and thus create a gene for a fusion between the 5' end of the target gene and the lacZ gene. Also inserted into exon 4, downstream of the COIN cassette but still in exon 4, was an FRT site flanked neomuycin resistance gene cassette and a splice acceptor site. This allele is conditional-ready and splits exon 4 into exon 4a and 4b which has no effect on the mature transcript. (J:240991)
Expression
In Mice Carrying this Mutation: 191 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Pdgfa Mutation:  20 strains or lines available
References
Original:  J:240991 Andrae J, et al., Characterization of platelet-derived growth factor-A expression in mouse tissues using a lacZ knock-in approach. PLoS One. 2014;9(8):e105477
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
03/18/2025
MGI 6.24
The Jackson Laboratory