Pdgfatm1Vlcg
Targeted Allele Detail
|
Symbol: |
Pdgfatm1Vlcg |
Name: |
platelet derived growth factor, alpha; targeted mutation 1, Velocigene |
MGI ID: |
MGI:5897007 |
Synonyms: |
Pdgfaex4COIN, Pdgfafl |
Gene: |
Pdgfa Location: Chr5:138961769-138983125 bp, - strand Genetic Position: Chr5, 77.65 cM
|
Alliance: |
Pdgfatm1Vlcg page
|
|
|
Allele Type: |
|
Targeted (Conditional ready, No functional change) |
Mutation: |
|
Insertion
|
|
|
Mutation details: Exon 4 was targeted with the insertion of a COIN (conditional by inversion) cassette. The cassette consists of a splice acceptor site and a LacZ gene with polyA site flanked by lox71 and lox66 sites in head-to-head orientation. The cassette is inserted in inverted orientation compared to the target gene. Upon cre-mediated recombination the modified loxP sites will mediate irreversible inversion of the cassette and thus create a gene for a fusion between the 5' end of the target gene and the lacZ gene. Also inserted into exon 4, downstream of the COIN cassette but still in exon 4, was an FRT site flanked neomuycin resistance gene cassette and a splice acceptor site. This allele is conditional-ready and splits exon 4 into exon 4a and 4b which has no effect on the mature transcript.
(J:240991)
|
|
|
|
Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
Carrying any Pdgfa Mutation: |
20 strains or lines available
|
|
Original: |
J:240991 Andrae J, et al., Characterization of platelet-derived growth factor-A expression in mouse tissues using a lacZ knock-in approach. PLoS One. 2014;9(8):e105477 |
All: |
6 reference(s) |
|