Gt(ROSA)26Sor^{tm1(CAG-birA,-EGFP)Joez}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-birA,-EGFP)Joez}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Zhaolan Zhou
• MGI ID: MGI:5896352
• Synonyms: R26^BirA, R26^cBirA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-birA,-EGFP)Joez} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:240794
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector is designed with, from 5' to 3', a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a floxed-STOP neomycin cassette and polyA sequence, a bacterial birA (biotin ligase) gene, an internal ribosome entry site (IRES), and an enhanced green fluorescence protein (EGFP). This targeting vector was inserted into the Gt(ROSA)26Sor locus. (J:240794)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 945 strains or lines available

References

• Original: J:240794 Wood KH, et al., Tagging methyl-CpG-binding domain proteins reveals different spatiotemporal expression and supports distinct functions. Epigenomics. 2016 Apr;8(4):455-73
• All: 2 reference(s)