Dusp5^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dusp5^{tm1d(KOMP)Wtsi}
• Name: dual specificity phosphatase 5; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5888995
• Synonyms: Dusp5^-(Exc)
• Gene: Dusp5 Location: Chr19:53517576-53530240 bp, + strand Genetic Position: Chr19, 47.98 cM
• Alliance: Dusp5^{tm1d(KOMP)Wtsi} page
• IMPC: Dusp5 gene page

Mutation origin

• Mutant Cell Line: EPD0575_3_B05
• Germline Transmission: Earliest citation of germline transmission: J:240637
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 53521813 of Chromosome 19 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 53522677. The critical exon 2 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked exon 2. (J:240637)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dusp5 Mutation: 17 strains or lines available

References

• Original: J:240637 Kutty RG, et al., Dual Specificity Phosphatase 5 Is Essential for T Cell Survival. PLoS One. 2016;11(12):e0167246
• All: 2 reference(s)