Gpa33^{tm1.1(Ctnnb1*)Ern}
Targeted Allele Detail

Summary

• Symbol: Gpa33^{tm1.1(Ctnnb1*)Ern}
• Name: glycoprotein A33 transmembrane; targeted mutation 1.1, Matthias Ernst
• MGI ID: MGI:5888902
• Synonyms: gpA33^deltaN-Bcat
• Gene: Gpa33 Location: Chr1:165957807-165994079 bp, + strand Genetic Position: Chr1, 73.49 cM
• Alliance: Gpa33^{tm1.1(Ctnnb1*)Ern} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226549
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Gpa33^{tm1.1(Ctnnb1*)Ern} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ctnnb1 (MGI:88276)	lacking the N-terminal 1-131 amino acids

• Mutation details: The targeting construct introduced downstream of the translation stop site a loxP site, IRES, neomycin resistance cassette, polyadenylation sequence, loxP site, IRES sequence, and FLAG-tagged mouse Ctnnb1 cDNA lacking the N-terminal domain amino acids 1-131. Cre-mediated recombination removed the selection/STOP cassette and allowed for expression of the hypomorphic inserted sequence to mimic human sporadic colon cancer. (J:226549)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpa33 Mutation: 139 strains or lines available

References

• Original: J:226549 Buchert M, et al., A hypermorphic epithelial beta-catenin mutation facilitates intestinal tumorigenesis in mice in response to compounding WNT-pathway mutations. Dis Model Mech. 2015 Nov 1;8(11):1361-73
• All: 1 reference(s)