Tg(SLC10A1)39Mvw
Transgene Detail

Summary

• Symbol: Tg(SLC10A1)39Mvw
• Name: transgene insertion 39, Micheal Wiles
• MGI ID: MGI:5883446
• Synonyms: hNTCP/BAC
• Transgene: Tg(SLC10A1)39Mvw Location: unknown
• Alliance: Tg(SLC10A1)39Mvw page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(SLC10A1)39Mvw expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  SLC10A1 (6554)

  Slc10a1

  (MGI:97379)

• Mutation details: BAC RP11-72J8 was modified to include a 982bp synthetic gene that includes 500 bp of ROSA26 sequence to allow in vivo linearization by the CRISPR/Cas9 complex. Specifically, the gene contains from 5' to 3': human SLC10A1 sequence, an FRT-flanked PGK-Neo cassette, 500 bp of ROSA26 sequence and human SLC10A1 sequence (SLC10A1 sequence totals 397 bp). Sequence analysis indicates that the ROSA26 sequence is 76,428 bp upstream of exon 1 of the SLC10A1 gene. Single guide RNAs are designed to target the ROSA26 locus and the RP11-72J8 BAC. (J:263861)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:263861 Winer BY, et al., Preclinical assessment of antiviral combination therapy in a genetically humanized mouse model for hepatitis delta virus infection. Sci Transl Med. 2018 Jun 27;10(447)
• All: 1 reference(s)