Smc5^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Smc5^{tm1d(KOMP)Wtsi}
• Name: structural maintenance of chromosomes 5; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5882566
• Synonyms: Smc5^del
• Gene: Smc5 Location: Chr19:23183815-23251261 bp, - strand Genetic Position: Chr19, 18.04 cM
• Alliance: Smc5^{tm1d(KOMP)Wtsi} page
• IMPC: Smc5 gene page

Mutation origin

• Mutant Cell Line: EPD0395_1_F05
• Germline Transmission: Earliest citation of germline transmission: J:240155
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 23240733 of Chromosome 19 upstream of exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 23241614. Exon 4 is thus flanked by loxP sites. A null/knockout allele was created by flp- and cre-mediated recombination to remove the lacZ sequence, neo cassette and the floxed exon 4. (J:240155)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Smc5 Mutation: 42 strains or lines available

References

• Original: J:240155 Pryzhkova MV, et al., Conditional mutation of Smc5 in mouse embryonic stem cells perturbs condensin localization and mitotic progression. J Cell Sci. 2016 Apr 15;129(8):1619-34
• All: 3 reference(s)