Pgam5^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pgam5^{tm1c(EUCOMM)Wtsi}
• Name: phosphoglycerate mutase family member 5; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5882559
• Synonyms: Pgam5^fl, Pgam5^flox
• Gene: Pgam5 Location: Chr5:110407001-110417765 bp, - strand Genetic Position: Chr5, 53.43 cM, cytoband F
• Alliance: Pgam5^{tm1c(EUCOMM)Wtsi} page
• IMPC: Pgam5 gene page

Mutation origin

• Mutant Cell Line: EPD0226_5_H02
• Germline Transmission: Earliest citation of germline transmission: J:240016
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 110414719 of Chromosome 5 upstream of the critical exon (exon 2) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 2 at position 110415572. Exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving exon 2 flanked by loxP sites. (J:240016)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pgam5 Mutation: 26 strains or lines available

References

• Original: J:240016 Moriwaki K, et al., The Mitochondrial Phosphatase PGAM5 Is Dispensable for Necroptosis but Promotes Inflammasome Activation in Macrophages. J Immunol. 2016 Jan 01;196(1):407-15
• All: 1 reference(s)