Tg(Flt4-Venus)7Tjl
Transgene Detail

Summary

• Symbol: Tg(Flt4-Venus)7Tjl
• Name: transgene insertion 7, Jean-Leon Thomas
• MGI ID: MGI:5882513
• Synonyms: Vegfr3::YFP
• Transgene: Tg(Flt4-Venus)7Tjl Location: unknown
• Alliance: Tg(Flt4-Venus)7Tjl page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The bacterial artificial chromosome (BAC) clone RP23-65D23, containing a ~238-kb mouse genomic fragment encompassing the FMS-like tyrosine kinase 4 (Flt4; also called Vegfr3) gene, was modified by insertion of the sequence encoding Venus yellow fluorescent protein (YFP), followed by a polyadenylation signal, immediatedly following the translation initiating ATG in the first exon. The modified BAC was injected into pronuclei of fertilized mouse oocytes. Expression specifically in VEGFR3-expressing cells is demonstrated by the correspondence of YFP fluorescence and immunohistochemical labeling using an anti-VEGFR3 antibody in retinal endothelial tip cells and lymphatic endothelial cells, but not arteries or veins of postnatal mice and of subventricular zone (SVZ) neurons along the striatal wall of the lateral ventricle in adult brain, as well as by selective expression of Flt4 mRNA in FACS-sorted YFP-positive cells of these mice. (J:171610)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:171610 Calvo CF, et al., Vascular endothelial growth factor receptor 3 directly regulates murine neurogenesis. Genes Dev. 2011 Apr 15;25(8):831-44
• All: 7 reference(s)