Gt(ROSA)26Sor^{tm10(CAG-Syp/EGFP*,-tdTomato)Dym}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm10(CAG-Syp/EGFP*,-tdTomato)Dym}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 10, Susan Dymecki
• MGI ID: MGI:5882396
• Synonyms: RC::FPSit, Rosa26FSF-LSL-Synaptophysin-GFP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm10(CAG-Syp/EGFP*,-tdTomato)Dym} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:240098
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: The targeting construct includes from 5' to 3' a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, an FRT-flanked STOP cassette, a loxP-flanked STOP cassette, a synaptophysin-GFP fusion gene (described below), IRES, a tdTomato gene, a WPRE, and a rabbit beta-globin polyA sequence. The synaptophysin-GFP fusion protein is a full-length mouse synaptophysin gene fused to a mutant enhanced green fluorescent protein (mut4EGFP; harboring the V163A/S175G codon changes for increased ability to fold properly at 37 degrees C). (J:240098)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1027 strains or lines available

References

• Original: J:240098 Niederkofler V, et al., Identification of Serotonergic Neuronal Modules that Affect Aggressive Behavior. Cell Rep. 2016 Nov 15;17(8):1934-1949
• All: 9 reference(s)