Ufl1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ufl1^{tm1c(EUCOMM)Wtsi}
• Name: UFM1 specific ligase 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5825300
• Synonyms: RCAD^F
• Gene: Ufl1 Location: Chr4:25248600-25281821 bp, - strand Genetic Position: Chr4, 10.59 cM, cytoband A3
• Alliance: Ufl1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Ufl1 gene page

Mutation origin

• Mutant Cell Line: EPD0169_3_F12
• Germline Transmission: Earliest citation of germline transmission: J:238253
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 25270175 of Chromosome 4 upstream of the critical exon 7 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 7 at position 25270175. The critical exon 7 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:238253)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ufl1 Mutation: 42 strains or lines available

References

• Original: J:238253 Zhang M, et al., RCAD/Ufl1, a Ufm1 E3 ligase, is essential for hematopoietic stem cell function and murine hematopoiesis. Cell Death Differ. 2015 Dec;22(12):1922-34
• All: 3 reference(s)