Tg(Myh6-DsRed1*/COX8A)40830Rag
Transgene Detail

Summary

• Symbol: Tg(Myh6-DsRed1*/COX8A)40830Rag
• Name: transgene insertion 4083, Roberta Gottlieb
• MGI ID: MGI:5824689
• Synonyms: alpha-MCH-MitoTimer
• Transgene: Tg(Myh6-DsRed1*/COX8A)40830Rag Location: unknown
• Alliance: Tg(Myh6-DsRed1*/COX8A)40830Rag page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Reporter)
• Mutation: Insertion
• Mutation details: The transgenic construct contains a murine Myh6 gene promoter directing expression of an a modified dsRed1 cDNA fused to the mitochondrial targeting sequence from human COX8A followed by a human growth hormone polyadenylation signal. Transgene expression is detected predominantly in the heart ventricles, with lower expression in the atria, which is expected due to the lower number of mitochondria in the atrial cardiomyocytes. The modified DsRed1-E5 (or Timer) fluorescent protein contains two amino acid substitutions, V105A and S197T, which increase fluorescence activity and allow a color shift from green to red over 48 hours. (J:238987)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:238987 Stotland A, et al., alpha-MHC MitoTimer mouse: In vivo mitochondrial turnover model reveals remarkable mitochondrial heterogeneity in the heart. J Mol Cell Cardiol. 2016 Jan;90:53-8
• All: 4 reference(s)