Nalcn^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Nalcn^{tm1c(KOMP)Wtsi}
• Name: sodium leak channel, non-selective; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5823777
• Synonyms: Nalcn^flox, NALCN^fx
• Gene: Nalcn Location: Chr14:123514046-123864556 bp, - strand Genetic Position: Chr14, 66.04 cM
• Alliance: Nalcn^{tm1c(KOMP)Wtsi} page
• IMPC: Nalcn gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238767
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 123829893 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 123830927. The "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving the critical exons flanked by loxP sites. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:238767)

Tumor Data

List all tumor models in MMHCdb carrying Nalcn^{tm1c(KOMP)Wtsi}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nalcn Mutation: 116 strains or lines available

References

• Original: J:238767 Flourakis M, et al., A Conserved Bicycle Model for Circadian Clock Control of Membrane Excitability. Cell. 2015 Aug 13;162(4):836-48
• All: 7 reference(s)