Pdx1^tm1.1Cvw
Targeted Allele Detail

Summary

• Symbol: Pdx1^tm1.1Cvw
• Name: pancreatic and duodenal homeobox 1; targeted mutation 1.1, Christopher VE Wright
• MGI ID: MGI:5823773
• Synonyms: Pdx1^deltaII
• Gene: Pdx1 Location: Chr5:147206907-147212658 bp, + strand Genetic Position: Chr5, 86.84 cM
• Alliance: Pdx1^tm1.1Cvw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238418
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Using the Pdx1^tm1Mgn Loxed Cassette Acceptor allele as a starting point, the cassette was replaced with another cassette via cre recombination mediated cassette exchange. The replacement cassette is comprised of: forward lox71 site, an FRT site flanked hygromycin resistance gene cassette, the genomic sequence encompassing the cis-regulatory area IV, I and III regions and exon 1, and a reverse lox2272 site. After removal of the hygro cassette through flp-mediated recombination this leaves an allele with a deletion of 256 bp upstream of exon 1 wich includes the entire area II. (J:238418)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pdx1 Mutation: 35 strains or lines available

References

• Original: J:238418 Yang YP, et al., The mammal-specific Pdx1 Area II enhancer has multiple essential functions in early endocrine cell specification and postnatal beta-cell maturation. Development. 2017 Jan 15;144(2):248-257
• All: 1 reference(s)