H2-Ab1^b-tm2Hpl
Targeted Allele Detail

Summary

• Symbol: H2-Ab1^b-tm2Hpl
• Name: histocompatibility 2, class II antigen A, beta 1; targeted mutation 2, Hidde Ploegh
• MGI ID: MGI:5819102
• Synonyms: H2-Ab1^tm2Hpl, I-A-beta-K225R-EGFP
• Gene: H2-Ab1 Location: Chr17:34482201-34488392 bp, + strand Genetic Position: Chr17, 17.98 cM
• Alliance: H2-Ab1^b-tm2Hpl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238328
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Reporter)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The endogenous class II MHC locus was replaced by a construct that encodes a mutant class II MHC beta-chain that cannot be ubiquitinated (K225R). A construct was used in which the long arm of homology corresponds to exons 24 of the I-Ab locus, followed by fused exons 5 and 6, in frame to the coding region for EGFP. The K225R mutation in the cytoplasmic tail of class II MHC was introduced into exon 4 of this construct. The construct contained a self-excisable ACN cassette that consists of a neomycin resistance gene, and a gene for Cre recombinase under the control of a sperm-specific promoter flanked by loxP sites. Transcriptional induction of the ACN cassette resulted in the excision of the neomycin resistance gene and Cre recombinase when the gene is transmitted through the male germline. (J:238328)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any H2-Ab1 Mutation: 81 strains or lines available

References

• Original: J:238328 McGehee AM, et al., Ubiquitin-dependent control of class II MHC localization is dispensable for antigen presentation and antibody production. PLoS One. 2011 Apr 20;6(4):e18817
• All: 2 reference(s)