Mpc2^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mpc2^{tm1c(EUCOMM)Wtsi}
• Name: mitochondrial pyruvate carrier 2; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5816915
• Synonyms: Mpc2^flox
• Gene: Mpc2 Location: Chr1:165288829-165308787 bp, + strand Genetic Position: Chr1, 72.8 cM, cytoband H2
• Alliance: Mpc2^{tm1c(EUCOMM)Wtsi} page
• IMPC: Mpc2 gene page

Mutation origin

• Mutant Cell Line: EPD0724_1_A12
• Germline Transmission: Earliest citation of germline transmission: J:236436
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 165305299 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 165306159. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:236436)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mpc2 Mutation: 14 strains or lines available

References

• Original: J:236436 McCommis KS, et al., Loss of Mitochondrial Pyruvate Carrier 2 in the Liver Leads to Defects in Gluconeogenesis and Compensation via Pyruvate-Alanine Cycling. Cell Metab. 2015 Oct 6;22(4):682-94
• All: 11 reference(s)