Reg4^{tm1(HBEGF/DsRed)Cle}
Targeted Allele Detail

Summary

• Symbol: Reg4^{tm1(HBEGF/DsRed)Cle}
• Name: regenerating islet-derived family, member 4; targeted mutation 1, Hans Clevers
• MGI ID: MGI:5816733
• Synonyms: delta neo Reg4-DTR-Red, Reg4^DTR-Red
• Gene: Reg4 Location: Chr3:98129472-98144064 bp, + strand Genetic Position: Chr3, 42.73 cM, cytoband F3
• Alliance: Reg4^{tm1(HBEGF/DsRed)Cle} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:235576
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Reg4^{tm1(HBEGF/DsRed)Cle} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HBEGF (1839)

  Hbegf

  (MGI:96070)

• Mutation details: A targeting vector containing a human HBEGF diphtheria-toxin receptor (DTR) cDNA linked in frame to the dsRed Express2 fluorescent protein by a 2A peptide, a FRT-flanked PGKneo cassette, a bovine growth hormone polyadenylation signal and two loxP sites, are used to disrupt the translation initiation codon in exon 2. DsRed fluorescence is detected in small intestine Paneth cells and colonic deep crypt secretory cells, and mimics the endogenous Reg4 gene expression pattern. No red fluorescence is detected in pancreas, stomach, or liver. (J:235576)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Reg4 Mutation: 22 strains or lines available

References

• Original: J:235576 Sasaki N, et al., Reg4+ deep crypt secretory cells function as epithelial niche for Lgr5+ stem cells in colon. Proc Natl Acad Sci U S A. 2016 Sep 13;113(37):E5399-407
• All: 1 reference(s)