Dpy30^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Dpy30^{tm1d(KOMP)Wtsi}
• Name: dpy-30, histone methyltransferase complex regulatory subunit; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5812162
• Synonyms: Dpy30^-
• Gene: Dpy30 Location: Chr17:74606469-74630939 bp, - strand Genetic Position: Chr17, 45.64 cM
• Alliance: Dpy30^{tm1d(KOMP)Wtsi} page
• IMPC: Dpy30 gene page

Mutation origin

• Mutant Cell Line: EPD0714_4_A06
• Germline Transmission: Earliest citation of germline transmission: J:237262
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 74614423 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 74615334. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exons. (J:237262)

Expression

In Mice Carrying this Mutation:

3 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dpy30 Mutation: 46 strains or lines available

References

• Original: J:237262 Yang Z, et al., Dpy30 is critical for maintaining the identity and function of adult hematopoietic stem cells. J Exp Med. 2016 Oct 17;213(11):2349-2364
• All: 4 reference(s)