Ddx60^{tm1d(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ddx60^{tm1d(EUCOMM)Wtsi}
• Name: DExD/H box helicase 60; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5806064
• Synonyms: "full" Ddx60^-
• Gene: Ddx60 Location: Chr8:62381121-62490735 bp, + strand Genetic Position: Chr8, 31.51 cM
• Alliance: Ddx60^{tm1d(EUCOMM)Wtsi} page
• IMPC: Ddx60 gene page

Mutation origin

• Mutant Cell Lines: EPD0327_7_A05, EPD0327_7_D07
• Germline Transmission: Earliest citation of germline transmission: J:236435
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 62407097 of Chromosome 8 upstream of exon 9 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 9 at position 62407909. Exon 9 thus flanked by loxP sites. A knockout allele was created by flp and cre recombinase expression in mice, removing the lacZ and neomycin cassette sequences and exon 9. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:236435)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ddx60 Mutation: 95 strains or lines available

References

• Original: J:236435 Goubau D, et al., Mouse superkiller-2-like helicase DDX60 is dispensable for type I IFN induction and immunity to multiple viruses. Eur J Immunol. 2015 Dec;45(12):3386-403
• All: 1 reference(s)