Tg(Myh6-SLC9A1*)8Gad
Transgene Detail

Summary

• Symbol: Tg(Myh6-SLC9A1*)8Gad
• Name: transgene insertion 8, Alain-Pierre Gadeau
• MGI ID: MGI:5804129
• Synonyms: KNHE1
• Transgene: Tg(Myh6-SLC9A1*)8Gad Location: unknown
• Alliance: Tg(Myh6-SLC9A1*)8Gad page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-SLC9A1*)8Gad expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  SLC9A1 (6548)

  Slc9a1

  (MGI:102462)

• Mutation details: Mice with this transgene overexpress a mutant form of human solute carrier family 9 (sodium/hydrogen exchanger), member A1 (SLC9A1; formerly NHE1) in the myocardium. The transgene contains a 5.5-kb mouse genomic DNA fragment that encompasses the promoter of the myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) gene driving expression of a 2551-bp cDNA encoding C-terminally hemagglutinin- (HA-) tagged human SLC9A1 in which four positively charged amino acids in the cytoplasmic regulatory domain (lysine at amino acid position 641 and arginine at positions 643, 645 and 647) have been replaced by the negatively-charged glutamic acid (K641E, R643E, R645E, R647E). These substitutions increase the protein's Na⁺ H⁺ transporter activity by shifting its pH preference toward the alkaline and by interfering with its regulation by calmoduolin. (J:82809, J:125924)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 4 reference(s)