Pmp2^{tm1.1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Pmp2^{tm1.1(KOMP)Vlcg}
• Name: peripheral myelin protein 2; targeted mutation 1, Velocigene
• MGI ID: MGI:5803681
• Synonyms: Pmp2^-, Pmp2^deltaEx1-4
• Gene: Pmp2 Location: Chr3:10244911-10248945 bp, - strand Genetic Position: Chr3, 2.54 cM
• Alliance: Pmp2^{tm1.1(KOMP)Vlcg} page
• IMPC: Pmp2 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:211344
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 3091bp between positions 10180726-10183816 of Chromosome 3 (Genome Build37). Exons 1-4 were replaced by a lacZ sequence (in frame after the original ATG) and a loxP-neo-loxP cassette. Cre-mediated recombination removed the neo cassette. RT-PCR and Western blot analyses confirmed the absence of mRNA and protein expression in sciatic nerve endoneurium of P56 homozygous mutant mice. Coimmunostaining of Pmp2 and Mbp on P10 sciatic nerve cross sections confirmed the loss of Pmp2 expression in all Schwann cells with no detectable changes in Mbp expression. (J:136110, J:211344)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pmp2 Mutation: 13 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 2 reference(s)