Kmo^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kmo^{tm1c(KOMP)Wtsi}
• Name: kynurenine 3-monooxygenase; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5796292
• Synonyms: Kmo^FRT-deleted, Kmo^{tm1c(KOMP)Wtsi/flox(ex5)}
• Gene: Kmo Location: Chr1:175459759-175488419 bp, + strand Genetic Position: Chr1, 81.6 cM
• Alliance: Kmo^{tm1c(KOMP)Wtsi} page
• IMPC: Kmo gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:233151
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 175470673 of Chromosome 1 upstream of exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 175471397. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, removing the lacZ sequences and the neomycin selection cassette. Exon 4 remains flanked by loxP sites. (J:233151)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kmo Mutation: 36 strains or lines available

References

• Original: J:233151 Mole DJ, et al., Kynurenine-3-monooxygenase inhibition prevents multiple organ failure in rodent models of acute pancreatitis. Nat Med. 2016 Feb;22(2):202-9
• All: 3 reference(s)