Rab4b^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rab4b^em1(IMPC)J
• Name: RAB4B, member RAS oncogene family; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:5795836
• Synonyms: Rab4b^em1J
• Gene: Rab4b Location: Chr7:26867858-26878308 bp, - strand Genetic Position: Chr7, 15.84 cM
• Alliance: Rab4b^em1(IMPC)J page
• IMPC: Rab4b gene page

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele from project Rab4b-7935J-M7105 was generated at The Jackson Laboratory by injecting Cas9 RNA and 4 guide sequences TAGCCTGGGTTCCCAAGGAG, ATGCTGAGACTAGATTCTCC, AGACCATCCACCAAAGCTAG and GAATTGGGGTACAGAAAAGG, which resulted in a 435 bp deletion beginning at Chromosome 7 negative strand position 27,176,049 bp GCTTGCCTCTAGCTTTGGTG, and ending after TCAGCTCCTGGAGAATCTAG at 27,175,615 bp (GRCm38/mm10). This mutation deletes exon 3 and 320 bp of flanking intronic sequence including the splice acceptor and donor, and is predicted to cause a change of amino acid sequence after residue 32 and early truncation 2 amino acids later. (J:188991)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rab4b Mutation: 18 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 3 reference(s)