Pnkp^tm2.1Pmc
Targeted Allele Detail

Summary

• Symbol: Pnkp^tm2.1Pmc
• Name: polynucleotide kinase 3'- phosphatase; targeted mutation 2.1, Peter J McKinnon
• MGI ID: MGI:5795746
• Synonyms: Pnkp^T424GfsX48
• Gene: Pnkp Location: Chr7:44506563-44512416 bp, + strand Genetic Position: Chr7, 28.94 cM, cytoband B2
• Alliance: Pnkp^tm2.1Pmc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226703
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: Initial targeting introduced a 17-bp mutation into exon 13 leaving a loxP site in the receding intron (between exons 12 and 13). A neomycin-thymidine kinase dual selection cassette flanked by frt sites with a single loxP was inserted between exons 3 and 4. Flp-mediated recombination removed the selection cassette. This mutation generates a frame-shift resulting in the insertion of an additional 48 aa at residue 385 and a premature stop codon and truncated protein. (J:226703)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pnkp Mutation: 38 strains or lines available

References

• Original: J:226703 Shimada M, et al., Polynucleotide kinase-phosphatase enables neurogenesis via multiple DNA repair pathways to maintain genome stability. EMBO J. 2015 Oct 1;34(19):2465-80
• All: 1 reference(s)