Zmiz1^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Zmiz1^{tm1c(EUCOMM)Hmgu}
• Name: zinc finger, MIZ-type containing 1; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:5795595
• Synonyms: Zmiz1^f
• Gene: Zmiz1 Location: Chr14:25459609-25667167 bp, + strand Genetic Position: Chr14, 15.03 cM
• Alliance: Zmiz1^{tm1c(EUCOMM)Hmgu} page
• IMPC: Zmiz1 gene page

Mutation origin

• Mutant Cell Line: HEPD0641_2_B09
• Germline Transmission: Earliest citation of germline transmission: J:234445
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 25644591 of Chromosome 14 upstream of exon 9 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the exon 9 at position 25645559. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, resulting in the deletion of the lacZ sequence and neomycin selection cassette. The exon 9 remains flanked by loxP sites. (J:234445)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zmiz1 Mutation: 40 strains or lines available

References

• Original: J:234445 Pinnell N, et al., The PIAS-like Coactivator Zmiz1 Is a Direct and Selective Cofactor of Notch1 in T Cell Development and Leukemia. Immunity. 2015 Nov 17;43(5):870-83
• All: 2 reference(s)