Wdfy3^{tm1c(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Wdfy3^{tm1c(KOMP)Mbp}
• Name: WD repeat and FYVE domain containing 3; targeted mutation 1c, Mouse Biology Program, UC Davis
• MGI ID: MGI:5795593
• Synonyms: Wdfy3^loxP
• Gene: Wdfy3 Location: Chr5:101980822-102217787 bp, - strand Genetic Position: Chr5, 48.95 cM
• Alliance: Wdfy3^{tm1c(KOMP)Mbp} page
• IMPC: Wdfy3 gene page

Mutation origin

• Mutant Cell Line: DEPD00523_5_G11
• Germline Transmission: Earliest citation of germline transmission: J:225200
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutation: Insertion Vector: L1L2_Bact_P Mutation details: The L1L2_Bact_P cassette was inserted at position 102100564 of Chromosome 5 upstream of the critical exon (exon 8) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 8 at position 102101479. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, resulting in the deletion of the lacZ sequence and neomycin selection cassette. Exon 8 remains flanked by loxP sites. (J:225200)

Generation of the Wdfy3^tm1a(KOMP)Mbp and Wdfy3^tm1c(KOMP)Mbp alleles

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wdfy3 Mutation: 210 strains or lines available

References

• Original: J:225200 Orosco LA, et al., Loss of Wdfy3 in mice alters cerebral cortical neurogenesis reflecting aspects of the autism pathology. Nat Commun. 2014;5:4692
• All: 3 reference(s)