Izumo1r^{tm2c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Izumo1r^{tm2c(KOMP)Wtsi}
• Name: IZUMO1 receptor, JUNO; targeted mutation 2c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5792910
• Synonyms: Juno^tm2c
• Gene: Izumo1r Location: Chr9:14797110-14815245 bp, - strand Genetic Position: Chr9, 4.42 cM
• Alliance: Izumo1r^{tm2c(KOMP)Wtsi} page
• IMPC: Izumo1r gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:209568
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 14811054 of Chromosome 9 upstream of the critical exons 4 and 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 5 at position 14812642. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, removing the lacZ sequences and neomycin selection cassette and leaving exons 4 and 5 flanked by loxP sites. (J:209568)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Izumo1r Mutation: 23 strains or lines available

References

• Original: J:209568 Bianchi E, et al., Juno is the egg Izumo receptor and is essential for mammalian fertilization. Nature. 2014 Apr 24;508(7497):483-7
• All: 1 reference(s)