Ccno^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ccno^{tm1b(EUCOMM)Wtsi}
• Name: cyclin O; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5792813
• Synonyms: Ccno^RA
• Gene: Ccno Location: Chr13:113124363-113127313 bp, + strand Genetic Position: Chr13, 63.93 cM
• Alliance: Ccno^{tm1b(EUCOMM)Wtsi} page
• IMPC: Ccno gene page

Mutation origin

• Mutant Cell Line: EPD0562_1_C09
• Germline Transmission: Earliest citation of germline transmission: J:220709
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 113125038 of Chromosome 13 upstream of the critical exons 2 and 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 113126827. The critical exons 2 and 3 are thus flanked by loxP sites. Cre expression without flp expression produces a reporter knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:220709)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ccno Mutation: 19 strains or lines available

References

• Original: J:220709 Funk MC, et al., Cyclin O (Ccno) functions during deuterosome-mediated centriole amplification of multiciliated cells. EMBO J. 2015 Apr 15;34(8):1078-89
• All: 1 reference(s)