Mecp2^tm3.1Joez
Targeted Allele Detail

Summary

• Symbol: Mecp2^tm3.1Joez
• Name: methyl CpG binding protein 2; targeted mutation 3.1, Zhaolan Zhou
• MGI ID: MGI:5790795
• Synonyms: Mecp2^T158M-Tavi, T158M
• Gene: Mecp2 Location: ChrX:73070198-73129296 bp, - strand Genetic Position: ChrX, 37.63 cM
• Alliance: Mecp2^tm3.1Joez page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:251147
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Epitope tag, Humanized sequence)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: A targeting construct was designed to insert a loxP-flanked neomycin (neo) resistance cassette upstream of exon 4 of the gene. A missense mutation T158M found in humans carrying the neurological disorder Rett Syndrome (RTT) was introduced to exon 4. A 23 amino acid Tavi tag, allowing MECP2 protein to be biotinylated in the presence of E. coli biotin ligase, BirA, was introduced immediately upstream of Mecp2 stop codon. Cre-mediated recombination removed the upstream floxed neo cassette. (J:251147)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mecp2 Mutation: 38 strains or lines available

References

• Original: J:251147 Johnson BS, et al., Biotin tagging of MeCP2 in mice reveals contextual insights into the Rett syndrome transcriptome. Nat Med. 2017 Oct;23(10):1203-1214
• All: 2 reference(s)