Nlk^{tm2c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Nlk^{tm2c(KOMP)Wtsi}
• Name: nemo like kinase; targeted mutation 2c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5788454
• Synonyms: Nlk-loxP
• Gene: Nlk Location: Chr11:78457994-78588199 bp, - strand Genetic Position: Chr11, 46.74 cM
• Alliance: Nlk^{tm2c(KOMP)Wtsi} page
• IMPC: Nlk gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:237770
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 78517165 of Chromosome 11 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2 at position 78518021. Exon 2 thus flanked by loxP sites. Flp-mediated recombination removed the FRT-flanked lacZ sequence and neo cassette. (J:237770)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nlk Mutation: 46 strains or lines available

References

• Original: J:237770 Liu R, et al., Nemo-Like Kinase (NLK) Is a Pathological Signaling Effector in the Mouse Heart. PLoS One. 2016;11(10):e0164897
• All: 2 reference(s)