Dip2a^em#bYwz
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dip2a^em#bYwz
• Name: disco interacting protein 2 homolog A; endonuclease-mediated mutation #b, Yaowu Zheng
• MGI ID: MGI:5787959
• Synonyms: Dip2a^LacZ
• Gene: Dip2a Location: Chr10:76098581-76181194 bp, - strand Genetic Position: Chr10, 38.76 cM
• Alliance: Dip2a^em#bYwz page

Mutation origin

• Strain of Origin: (C57BL/6 x DBA/2)F1 x C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: CRISPR/Cas9 technology inserted between the 5' UTR and the exon 1 coding sequence a lacZ reporter gene with a nuclear localization sequence, a polyadenylation sequence and an FRT-flanked neomycin resistance cassette. Clones 4 (13 bp deletion), 6 (6 bp deletion), 11 (162 bp deletion), 12 (26 bp deletion), 14 (no deletion) and 17 (7 bp deletion) were generated. Clones 5 and 11 (represented by #) are positive for lacZ insertion. (J:232970)

Expression

In Mice Carrying this Mutation:

116 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dip2a Mutation: 71 strains or lines available

References

• Original: J:232970 Zhang L, et al., Large genomic fragment deletions and insertions in mouse using CRISPR/Cas9. PLoS One. 2015;10(3):e0120396
• All: 3 reference(s)