Ptger3^tm1.1Brey
Targeted Allele Detail

Summary

• Symbol: Ptger3^tm1.1Brey
• Name: prostaglandin E receptor 3 (subtype EP3); targeted mutation 1.1, Richard M Breyer
• MGI ID: MGI:5784744
• Synonyms: EP3^-
• Gene: Ptger3 Location: Chr3:157272459-157350392 bp, + strand Genetic Position: Chr3, 81.32 cM
• Alliance: Ptger3^tm1.1Brey page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232822
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting vector contained a loxP-flanked neo selection cassette in intron 1 and the exon coding for the beta C-terminal variant replacing the gamma variant specific sequence at the 3' end of exon 2. A third loxP site was placed downstream of the beta exon. Subsequently, cre-mediated recombination removed the neo cassette and the remainder of exon 2 and the beta exon, creating an allele that knocks out all three (alpha, beta, gamma) splice variants. (J:232822)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ptger3 Mutation: 22 strains or lines available

References

• Original: J:232822 Ceddia RP, et al., The PGE2 EP3 Receptor Regulates Diet-Induced Adiposity in Male Mice. Endocrinology. 2016 Jan;157(1):220-32
• All: 3 reference(s)