Gt(ROSA)26Sor^{tm1(CAG-GLRA1*,-GFP)Yqd}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-GLRA1*,-GFP)Yqd}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Yu-qiang Ding
• MGI ID: MGI:5784524
• Synonyms: Rosa26-IVMR
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-GLRA1*,-GFP)Yqd} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232456
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129 x C57BL/6

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-GLRA1*,-GFP)Yqd} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  GLRA1 (2741)

  Glra1

  (MGI:95747)

  with mutations F207A and A288G

• Mutation details: The targeting construct inserted into the locus a CAG promoter, floxed STOP cassette, ivermectin (IVM)-gated silencing receptor (IVMR) containing the human GLRA1 coding sequence with two amino acid substitutions (F207A and A288G), a 2A cleavage site, GFP, WPRE (the woodchuck hepatitis virus posttranscriptional regulatory element), polyadenylation sequence, and neomycin resistance cassette. This allele allows for inducible and reversible silencing neurons. (J:232456)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:232456 Hu L, et al., A mouse line for inducible and reversible silencing of specific neurons. Mol Brain. 2014;7:68
• All: 1 reference(s)