Enho^tm1.1Butl
Targeted Allele Detail

Summary

• Symbol: Enho^tm1.1Butl
• Name: energy homeostasis associated; targeted mutation 1.1, Andrew A Butler
• MGI ID: MGI:5781010
• Synonyms: AdrKO
• Gene: Enho Location: Chr4:41638144-41640302 bp, - strand Genetic Position: Chr4, 21.8 cM, cytoband B1
• Alliance: Enho^tm1.1Butl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:225402
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted upstream of exon 2. An additional loxP site was inserted in exon 2 downstream of the coding sequence. Cre-mediated recombination removed the loxP-flanked region, including the coding sequence and the selection cassette. RT-PCR analysis revealed absence of RNA expression in the liver, whole brain, and skeletal muscle of homozygous mutant mice. Absence of serum levels in chow-fed conditions was confirmed by ELISA. (J:225402)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Enho Mutation: 6 strains or lines available

References

• Original: J:225402 Ganesh Kumar K, et al., Adropin deficiency is associated with increased adiposity and insulin resistance. Obesity (Silver Spring). 2012 Jul;20(7):1394-402
• All: 2 reference(s)