Themis2^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Themis2^{tm1d(KOMP)Wtsi}
• Name: thymocyte selection associated family member 2; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5779207
• Gene: Themis2 Location: Chr4:132509148-132523675 bp, - strand Genetic Position: Chr4, 65.9 cM
• Alliance: Themis2^{tm1d(KOMP)Wtsi} page
• IMPC: Themis2 gene page

Mutation origin

• Mutant Cell Line: EPD0031_1_C01
• Germline Transmission: Earliest citation of germline transmission: J:232061
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 132512240 of Chromosome 4 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon 4 at position 132514050. The critical exon 4 is thus flanked by loxP sites. Flp- and cre-mediated recombination resulted in a knockout mouse by removal of the reporter cassette and exon 4. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:232061)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Themis2 Mutation: 26 strains or lines available

References

• Original: J:232061 Hartweger H, et al., Themis2 is not required for B cell development, activation, and antibody responses. J Immunol. 2014 Jul 15;193(2):700-7
• All: 1 reference(s)