Map1a^{tm1.2(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Map1a^{tm1.2(KOMP)Mbp}
• Name: microtubule-associated protein 1 A; targeted mutation 1.2, Mouse Biology Program, UC Davis
• MGI ID: MGI:5774957
• Synonyms: Map1a^tm1.2
• Gene: Map1a Location: Chr2:121120081-121141313 bp, + strand Genetic Position: Chr2, 60.37 cM
• Alliance: Map1a^{tm1.2(KOMP)Mbp} page
• IMPC: Map1a gene page

Mutation origin

• Mutant Cell Line: DEPD00515_2_A07
• Germline Transmission: Earliest citation of germline transmission: J:220153
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The insertion of the L1L2_Bact_P cassette created a deletion of size 8113 starting at position 121129742 and ending at position 121137855 of Chromosome 2 (Genome Build GRCm39). This deletion results in the removal of functionally critical exon 5. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Flp-mediated recombination removed the reporter and selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:220153)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Map1a Mutation: 109 strains or lines available

References

• Original: J:220153 Liu Y, et al., Mutations in the Microtubule-Associated Protein 1A (Map1a) Gene Cause Purkinje Cell Degeneration. J Neurosci. 2015 Mar 18;35(11):4587-98
• All: 1 reference(s)