Gpa33^tm1Jkhh
Targeted Allele Detail

Summary

• Symbol: Gpa33^tm1Jkhh
• Name: glycoprotein A33 transmembrane; targeted mutation 1, Joan K Heath
• MGI ID: MGI:5774782
• Gene: Gpa33 Location: Chr1:165957807-165994079 bp, + strand Genetic Position: Chr1, 73.49 cM
• Alliance: Gpa33^tm1Jkhh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:231808
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting construct replaced 20 kb of endogenous sequence from 5' end of exon 2 to the 5' end of exon 7 with a STOP cassette, IRES, beta-geo and polyadenylation sequence. RNA blotting confirmed the absence of transcript expression in intestinal epithelial cells. Immunohistochemistry confirmed the absence of protein expression in the small intestine and colon. (J:224768, J:231808)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gpa33 Mutation: 137 strains or lines available

References

• Original: J:231808 Pereira-Fantini PM, et al., A33 antigen-deficient mice have defective colonic mucosal repair. Inflamm Bowel Dis. 2010 Apr;16(4):604-12
• All: 2 reference(s)