Gt(ROSA)26Sor^tm1Acoh
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm1Acoh
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Adam Cohen
• MGI ID: MGI:5771697
• Synonyms: Ai130, Floxopatch
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm1Acoh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236369
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: A cassette containing pCAG-FRT-loxp-Stop codons-3x SV40 polyA-loxP-Optopatch2-WPRE (woodchuck hepatitis post-transcriptional regulatory element)-bGHpA (bovine growth hormone polyadenylation signal)-AttB-pPGK-FRT-neo-pA-AttP was introduced to intron 1. Optopatch2 is bicistronic construct that coexpresses QuasAr2-dark mOrange2 (an enhanced archaerhodopsin-3 (Arch)-based voltage indicator) and CheRiff-EGFP (a sensitive blue-shifted optogenetic actuator) separated by the porcine teschovirus-1 (P2A) peptide. This peptide causes a ribosomal skip, leading to near-balanced co-expression of the QuasAr2-dark mOrange2 and CheRiff-EGFP upon cre-mediated excision of the floxed Stop cassette. (J:236369)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:236369 Lou S, et al., Genetically Targeted All-Optical Electrophysiology with a Transgenic Cre-Dependent Optopatch Mouse. J Neurosci. 2016 Oct 26;36(43):11059-11073
• All: 1 reference(s)