Gt(ROSA)26Sor^{tm3(CAG-CHRM4*,-tdTomato)Ghsh}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3(CAG-CHRM4*,-tdTomato)Ghsh}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Anirvan Ghosh
• MGI ID: MGI:5770778
• Synonyms: R26^{ds-hM4D-tdTomato}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3(CAG-CHRM4*,-tdTomato)Ghsh} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226610
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: A targeting vector, which contained the CAG promoter, a loxP site flanked STOP fragment, an FRT site flanked STOP fragment, the human muscarinic acetylcholine receptor subtype 4 DREADD (hM4D) and tdTomato, was inserted into the locus via homologous recombination. The hM4D sequence is a G-coupled human M4 muscarinic DREADD (designer receptor exclusively activated by designer drug). To create the hM4D sequence, the wild-type human muscarinic 4 receptor (CHRM4) sequence was modified via site-directed mutagenesis to harbor two amino acid substitutions (Y113C<3.33>/A203G<5.46>) that abolish receptor affinity for the native ligand, acetylcholine (ACh), but allow receptor binding and subsequent activation by the small drug-like molecule clozapine-N-oxide (CNO). (J:226610)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1084 strains or lines available

References

• Original: J:226610 Bourane S, et al., Gate control of mechanical itch by a subpopulation of spinal cord interneurons. Science. 2015 Oct 30;350(6260):550-4
• All: 4 reference(s)