Gt(ROSA)26Sor^{tm1(CAG-Twnk*K320E,-EGFP)Rfjw}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Twnk*K320E,-EGFP)Rfjw}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Rudolf J Wiesner
• MGI ID: MGI:5770138
• Synonyms: Gt(ROSA)26Sor^{tm1(CAG-Peo1*K320E,-EGFP)Rfjw}, R26-K320E-Twinkle^loxP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Twnk*K320E,-EGFP)Rfjw} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:225849
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Inserted expressed sequence, Reporter)
• Mutations: Insertion, Nucleotide substitutions
• Gt(ROSA)26Sor^{tm1(CAG-Twnk*K320E,-EGFP)Rfjw} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Twnk (MGI:2137410)	K320E mutation

• Mutation details: The targeting construct introduced into the locus a CAG promoter, floxed neomycin/STP cassette, modified murine cDNA in which nucleotide substitutions results in the amino acid substitution of glutamic acid for lysine at position 320 (K320E), and an FRT-flanked IRES-EGFP reporter gene. (J:225849)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:225849 Baris OR, et al., Mosaic Deficiency in Mitochondrial Oxidative Metabolism Promotes Cardiac Arrhythmia during Aging. Cell Metab. 2015 May 5;21(5):667-77
• All: 11 reference(s)