Folh1^tm2Nle
Targeted Allele Detail

Summary

• Symbol: Folh1^tm2Nle
• Name: folate hydrolase 1; targeted mutation 2, Joseph H Neale
• MGI ID: MGI:5766053
• Synonyms: GCPII^-, GCPII KO
• Gene: Folh1 Location: Chr7:86368185-86425151 bp, - strand Genetic Position: Chr7, 48.51 cM, cytoband D1-D2
• Alliance: Folh1^tm2Nle page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:224551
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced exon 3 through 5. Western blot analysis confirmed the absence of protein expression in the brain of homozygous mutant mice. The residual N-acetylaspartylglutamate (NAAG) hydrolyzing activity in the brain was ~6% of that seen in wild-type controls. (J:224551)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Folh1 Mutation: 50 strains or lines available

References

• Original: J:224551 Gao Y, et al., Mice lacking glutamate carboxypeptidase II develop normally, but are less susceptible to traumatic brain injury. J Neurochem. 2015 Jul;134(2):340-53
• All: 2 reference(s)