Hook3^{tm1a(NCOM)Mfgc}
Targeted Allele Detail

Summary

• Symbol: Hook3^{tm1a(NCOM)Mfgc}
• Name: hook microtubule tethering protein 3; targeted mutation 1a, Mammalian Functional Genomics Centre
• MGI ID: MGI:5766016
• Gene: Hook3 Location: Chr8:26511449-26609252 bp, - strand Genetic Position: Chr8, 14.29 cM
• Alliance: Hook3^{tm1a(NCOM)Mfgc} page
• IMPC: Hook3 gene page

Mutation origin

• Mutant Cell Lines: M00033_C_375W_B05, M00033_C_375W_D01, M00033_C_375W_D04, M00033_C_375W_F05
• Germline Transmission: Earliest citation of germline transmission: J:165964
• Parent Cell Line: C2 (Tcp) (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: NorCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion Vector: L1L2_MFGC3
• Mutation details: The NorCOMM2LS selection cassette replaces the region between positions 26071677 and 26071682 of Chr 8 upstream of the targeted exon(s) (Build 38). The cassette has an F3 site followed by AttB1, IRES and lacZ sequences and then a loxP site. This first loxP site is followed by TK1, a T2A sequence and a human beta-actin promoter neomycin PGK polyA selection cassette. These are followed by a second F3 site and a second loxP site. A third loxP cassette is inserted downstream of the targeted exon(s) between positions 26069251 and 26069270 with the third loxP followed by an FRT site and AttB2 sequence. The targeted critical exon(s) is(are) thus flanked by loxP sites. A floxed allele can be created by flp recombination. Subsequent cre excision results in a knockout mouse. If cre excision occurs before flp excision, a reporter knockout mouse will be created. See http://www.mousephenotype.org/data/genes/MGI:2443554 for more information. (J:165964)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hook3 Mutation: 120 strains or lines available

References

• Original: J:165964 Mammalian Functional Genomics Centre, Alleles produced for the NorCOMM project by the Mammalian Functional Genomics Centre (Mfgc), University of Manitoba. MGI Direct Data Submission. 2010
• All: 1 reference(s)