Crkl^tm1.1Bem
Targeted Allele Detail

Summary

• Symbol: Crkl^tm1.1Bem
• Name: Crk like proto-oncogene, adaptor protein; targeted mutation 1.1, Bernice Morrow
• MGI ID: MGI:5763398
• Synonyms: Crkl-null
• Gene: Crkl Location: Chr16:17269851-17305298 bp, + strand Genetic Position: Chr16, 10.82 cM
• Alliance: Crkl^tm1.1Bem page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:230261
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 1. A floxed neomycin resistance cassette was inserted downstream of exon 1. Cre-mediated recombination removed exon 1 and a selection cassette. RT-PCR confirmed the absence of protein expression in E10.5 hearts. (J:230261)

Expression

In Mice Carrying this Mutation:

11 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crkl Mutation: 16 strains or lines available

References

• Original: J:230261 Racedo SE, et al., Mouse and human CRKL is dosage sensitive for cardiac outflow tract formation. Am J Hum Genet. 2015 Feb 5;96(2):235-44
• All: 2 reference(s)