Tg(HBB/lambda*/Rr27*)1Keij
Transgene Detail

Summary

• Symbol: Tg(HBB/lambda*/Rr27*)1Keij
• Name: transgene insertion 1, Keiji Tanimoto
• MGI ID: MGI:5762975
• Synonyms: deltaSOM TgM
• Transgene: Tg(HBB/lambda*/Rr27*)1Keij Location: unknown
• Alliance: Tg(HBB/lambda*/Rr27*)1Keij page

Transgene origin

• Strain of Origin: CD-1

Transgene description

• Transgene Type: Transgenic (Not Applicable)
• Mutation: Insertion
• Mutation details: The human AB775805 YAC was modified with the insertion into the 3'UTR of the HBB locus control region loxP5171 and loxP2272-flanked inverted copies of: 1) a CpG-rich 2.3 kb lambda bacteriophage DNA fragment with four CTCF-binding sites and fragment b (LCb) sequences from the H19 imprinting control region (ICR; Rr27), and 2) the H19 ICR lacking 37 bp of the Sox-Oct motifs (SOM) in fragment b. Lines 1 and 112 were generated. Line 1 was selected as the representative line. Cre-mediated recombination creates two transgenes: Tg(HBB,Rr27*)1dSOMKeij with the ICR lacking 37 bp in the SOM sequence, and Tg(HBB,lambda*)1LCbKeij with the LCb. (J:202262)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:202262 Sakaguchi R, et al., Sox-Oct motifs contribute to maintenance of the unmethylated H19 ICR in YAC transgenic mice. Hum Mol Genet. 2013 Nov 15;22(22):4627-37
• All: 1 reference(s)