Plin2^{tm1.1(cre/ERT2)Mort}
Targeted Allele Detail

Summary

• Symbol: Plin2^{tm1.1(cre/ERT2)Mort}
• Name: perilipin 2; targeted mutation 1.1, Rory E Morty
• MGI ID: MGI:5755034
• Gene: Plin2 Location: Chr4:86566623-86588297 bp, - strand Genetic Position: Chr4, 40.69 cM
• Alliance: Plin2^{tm1.1(cre/ERT2)Mort} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:250476
• Parent Cell Line: v6.5 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Recombinase, Reporter)
• Inducer: tamoxifen
• Mutation: Insertion
• Plin2^{tm1.1(cre/ERT2)Mort} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Plin2 (MGI:87920)

• Mutation details: A promoterless mCherry reporter gene, two T2A sequence and inducible cre recombinase fusion gene (cre/ERT2) were inserted into the exon 8 in-frame upstream of the endogenous Plin2 stop codon by homologous recombination using a targeting vector encoding 6 kb 50 and 3.6 kb 30 homologous sequences. Two T2A peptide sequences separate Plin2 and mCherry and CreERT2 from each other. The targeting vector also contained a diptheria toxin A cassette for negative and a FRT-flanked PGK-neomycin cassette downstream of the mCherry-CreERT2 cassette for positive selection. Chimeric males were backcrossed onto C57BL/6J background to achieve germline transmission and bred with a mouse expressing Flp to remove the FRT-PGK-neomycin selection cassette. (J:229959, J:250476)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Plin2 (mouse) Summary of all recombinase alleles driven by Plin2. Alliance mouse Plin2 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Plin2 Mutation: 37 strains or lines available

References

• Original: J:229959 Morty RE, Direct Data Submission for a Plin2 allele. MGI Direct Data Submission. 2016
• All: 3 reference(s)