Spp1^tm1Cant
Targeted Allele Detail

Summary

• Symbol: Spp1^tm1Cant
• Name: secreted phosphoprotein 1; targeted mutation 1, Harvey Cantor
• MGI ID: MGI:5752394
• Synonyms: Spp1^flstop
• Gene: Spp1 Location: Chr5:104582984-104588916 bp, + strand Genetic Position: Chr5, 50.68 cM
• Alliance: Spp1^tm1Cant page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:222609
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 45 nucleotides in exon 2 after the start codon that encode an N-terminal signal sequence were deleted. A floxed stop and neomycin resistance cassette was inserted upstream of the modified exon 2. Cre-mediated recombination is needed to achieve expression of the intracellular isoform. Western blot analysis confirmed the absence of protein expression (intracellular or soluble isoforms) in splenocyte. (J:222609)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spp1 Mutation: 44 strains or lines available

References

• Original: J:222609 Leavenworth JW, et al., A p85alpha-osteopontin axis couples the receptor ICOS to sustained Bcl-6 expression by follicular helper and regulatory T cells. Nat Immunol. 2015 Jan;16(1):96-106
• All: 4 reference(s)