Agrp^{tm1.1(cre/EGFP)Stei}
Targeted Allele Detail

Summary

• Symbol: Agrp^{tm1.1(cre/EGFP)Stei}
• Name: agouti related neuropeptide; targeted mutation 1.1, Robert A Steiner
• MGI ID: MGI:5752370
• Synonyms: Agrp-CreEGFP
• Gene: Agrp Location: Chr8:106293327-106306477 bp, - strand Genetic Position: Chr8, 53.04 cM, cytoband D1-D2
• Alliance: Agrp^{tm1.1(cre/EGFP)Stei} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:221577
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Epitope tag, Recombinase, Reporter)
• Mutation: Insertion
• Agrp^{tm1.1(cre/EGFP)Stei} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Agrp (MGI:892013)

• Mutation details: The targeting construct introduced upstream of the start codon a cre recombinase gene fused with EGFP (cre/EGFP), a myc tag, nuclear localization signal at the N-terminus and polyadenulation sequence. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette introduced downstream of the fusion gene. (J:221577)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Agrp (mouse) Summary of all recombinase alleles driven by Agrp. Comparative matrix view of recombinase activities Alliance mouse Agrp gene page

Expression

In Mice Carrying this Mutation:

1 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Agrp Mutation: 18 strains or lines available

References

• Original: J:221577 Sanz E, et al., Fertility-regulating Kiss1 neurons arise from hypothalamic POMC-expressing progenitors. J Neurosci. 2015 Apr 8;35(14):5549-56
• All: 8 reference(s)