Cdk5rap1^tm1.2Tomik
Targeted Allele Detail

Summary

• Symbol: Cdk5rap1^tm1.2Tomik
• Name: CDK5 regulatory subunit associated protein 1; targeted mutation 1.2, Kazuhito Tomizawa
• MGI ID: MGI:5750452
• Gene: Cdk5rap1 Location: Chr2:154177300-154214930 bp, - strand Genetic Position: Chr2, 76.46 cM, cytoband H2
• Alliance: Cdk5rap1^tm1.2Tomik page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:220703
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 5 and an FRT flanked neo cassette and loxP site were inserted downstream of exon 6 via homologous recombination. Recombinase mediated recombination removed exons 5-6 and the neo cassette. qPCR analysis of total RNAs isolated from brain, liver, skeletal muscle and heart tissues confirmed the absence of mRNA transcripts in homozygous mutant mice. (J:220703)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdk5rap1 Mutation: 109 strains or lines available

References

• Original: J:220703 Wei FY, et al., Cdk5rap1-mediated 2-methylthio modification of mitochondrial tRNAs governs protein translation and contributes to myopathy in mice and humans. Cell Metab. 2015 Mar 3;21(3):428-42
• All: 2 reference(s)